rabbit anti bmi1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rabbit anti bmi1
Rabbit Anti Bmi1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+bmi1/Bmi1+Rabbit+mAb/pmc12919260-306-7-9
Average 95 stars, based on 91 article reviews
Rabbit Anti Bmi1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+bmi1/Bmi1+Rabbit+mAb/pmc12919260-306-7-9
Average 95 stars, based on 91 article reviews
rabbit anti bmi1 - by Bioz Stars,
2026-09
95/100 stars
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ChIP-sequencing:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Immunofluorescence:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Transfection:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Concentration Assay:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Labeling:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Knockdown:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Western Blot:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Real-time Polymerase Chain Reaction:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Expressing:Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit anti-Activated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit anti-P19 ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence. Article Snippet: Slides were counterstained with DAPI and mounted using antifade mounting media.Slides were counterstained with DAPI and mounted using antifade mounting media.. Primary antibodies were used as follows: Rabbit anti-H2AK119ub (Cell Signaling, 8240S, 1:500), Rabbit anti-H3K4me3 (Abcam, ab8580, 1:1000), Rabbit anti-H3K27me3 (Cell Signaling, 9733S, 1:500), Rat anti-CD34 (eBiosciences, 14-0341-82, 1:100), Rabbit anti-SOX9 (Abcam, ab185966, 1:200), Rabbit antiActivated CASAPASE 3 (R&D systems, AF835, 1:100), Rabbit antiP19ARF (Abcam, ab80, 1:200), Rabbit anti-RING1B (Cell Signaling, 5694S, 1:100), and Rabbit anti-BMI1 ( Article Title: BMI1-mediated heterochromatinization represses G-quadruplex DNA formation to maintain genomic stability during replication Article Snippet: Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.Subsequently, membranes were blocked for 1h in 5% non-fat milk-1X TBS solution and incubated overnight with primary antibodies.. The antibodies used in this study are rabbit anti-BMI1 ( Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction. Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Article Snippet: The membranes were visualized using the SuperSignal detection system (Thermo Fisher Scientific, Prod 34080). the image was quantified using Adobe Photoshop CS6.. Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); |
![Tumor pathologic classification after 4-NQO treatment for 4 weeks reveals more oncogenic changes in mice with ectopic <t>BMI1</t> expression. A, Schematic of the DOX-inducible expression system (KrTB) used to overexpress ectopic BMI1 in murine tongue epithelial basal stem cells. B, Timeline outlining the different treatment groups for this experiment. Samples were collected at 10 or 16 weeks (4- or 10-week treatments). Samples from bolded groups [KrTB-DN (4w) and KrTB-DN (10w)] were also submitted for RNA-seq. C, Representative images of H&E-stained sections for normal tongue epithelium, hyperplasia, and dysplasia (100× magnification; scale bar, 100 μm). D, Graph of the distribution (%) of the most severe 4-NQO–induced lesion observed in each mouse by pathologic classification (at 4 weeks), with Kr-DN (4w) ( N = 7) and KrTB-DN (4w) ( N = 10) mice. E , Graph of the distribution (%) of the most severe 4-NQO–induced lesion observed in each mouse by pathologic classification (at 10 weeks), with Kr-DN (10w) ( N = 7) and KrTB-DN (10w) ( N = 10) mice. For D and E , statistical significance was determined with the χ 2 test. ****, P < 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6948/pmc12816948/pmc12816948__crc-25-0580_f1.jpg)
